# GUI overview The application is organized into tabs: ## Image Simulation Generate synthetic stacks for testing RICS/SFCS analysis. Common parameters: - **Image shape** (pixels) - **Number of frames** - **Pixel dwell time (µs)** and **pixel size (nm)** - **Number of particles**, **brightness**, **diffusion coefficients** - **PSF sigma** Outputs are saved as TIFF stacks. ## RICS Export Compute RICS correlation maps and uncertainty maps from an input stack. Inputs: - Single file or batch folder - Channel selection Key parameters: - **Crop factor**: fraction of the field used for correlation (reduces edge artefacts). - **Window size**: local window size (odd integer). - **Correct drift**: optional drift correction before correlation. ## RICS Fitting Fit diffusion models to the exported RICS correlation map. Key parameters: - **Pixel size (nm)** - **Pixel dwell (µs)** - **Line time (ms)** - **PSF size XY (µm)** and **aspect ratio** - **Diffusion model**: 2D, 3D, 2-component (depending on your implementation) - Optional **1D fast axis fit** Batch fitting is supported via a folder selection. ## SFCS Compute perpendicular scanning FCS autocorrelation curves. Key parameters: - Channel - Number of CPU cores - Optional bleach correction ## Results & Logs Contains the internal log output, plus plot export and session save/load features.