# Workflow: Diffusion map ## Purpose Estimate spatially varying diffusion parameters by fitting local regions of the data. ## Steps 1. Open the **RICS Fitting** tab. 2. In the **Diffusion Map Fitting Parameters** section: - Select input file - Choose channel - Set window size and offset 3. Click **Generate Diffusion Map**. ## Outputs Typical outputs include: - A diffusion map array (D map) - Auxiliary arrays (brightness/number maps if computed) - An auxiliary NPZ with intermediate results